Macrophage phagocytosis of polyethylene particulate in vitro

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National Library of Canada , Ottawa
SeriesCanadian theses = -- Thèses canadiennes
The Physical Object
Pagination2 microfiches : negative. --
ID Numbers
Open LibraryOL21330513M
ISBN 100612516040

In this study, mature human monocyte-derived macrophages (MDM) were adapted to a previously established in vitro model to examine the influence of high-density polyethylene (HDPE) particulate (4. For example, phagocytosis of nanoparticles made from human serum albumin of μm in diameter was higher than that of nm in diameter.

Recently, it has been found that polyethylene glycol-distearate-incorporated microspheres can modify the extent of the phagocytosis depending on the concentration of the excipient used. The amount of Cited by: Gene Expression Profile of Macrophage-Like U Cells in Response to Polyethylene Particles: The effect of polyethylene particle chemistry on human monocyte-macrophage function in vitro.

J.P. Santerre, A. Hinek, et hage phagocytosis of polyethylene particulate in by: 9. In Vitro Macrophage Phagocytosis Assay. January ; DOI: / and a method for the in vitro study of macrophage phagocytosis of IgG-opsonized or IgM/complement component 3-opsonized.

Due to their role in phagocytosis, macrophages are involved in many diseases of the immune system. For example, they participate in the formation of granulomas, inflammatory lesions that may be caused by a large number of diseases.

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Some disorders, mostly rare, of ineffective phagocytosis and macrophage function have been described, for on: Phagocytosis. In this work, we investigate the role of macrophage integrins Mac-1 and RGD-binding integrins in response to UHMWPE wear particles.

Using integrin knockout mice as well as integrin blocking techniques, reduction in macrophage phagocytosis and inflammatory cytokine secretion is demonstrated when these receptors are either absent or by: Modification of particle surfaces with polyethylene glycol and poloxamine is routinely used to reduce phagocytosis by postponing opsonization, or adsorption of proteins which increase phagocytosis.

However, an in depth understanding of the Macrophage phagocytosis of polyethylene particulate in vitro book of physical parameters and the biophysical origins of these roles is still by: Acknowledgments.

We thank Simon Bartlett for English editing. M.R.H. is supported by an FPI predoctoral fellowship from the Spanish Government (BES) and R.V-B. by a Juan de la Cierva postdoctoral contract from the Spanish Government (JCI). Work in V.A.’s laboratory is supported by grants from the Spanish Ministry of Economy and Competitivity (MINECO) (SAF Cited by: 5.

Phagocytosis assay. Collect peritoneal fluid of mice by injecting 5 mL of DMEM Hams F medium (without phenol red) in peritoneal cavity by following a gentle massage of the abdomen. Peritoneal exudate cell suspension (as a source of macrophages) may be used for in vitro phagocytosis assays.

Breeds Phagocytosis (%) Phagocytic index Phagocytic product a,b The numerical values within a column not sharing a common letter were significantly different (P Cited by: T1 - In vitro measurement of phagocytosis and killing of Cryptococcus neoformans by macrophages.

AU - Nicola, André Moraes. AU - Casadevall, Arturo. PY - /2/1. Y1 - /2/1. N2 - Macrophages are pivotal cells in immunity against a wide range of by: Macrophage phagocytosis inhibition by mast cells is dependent on TLR4.

(A) Representative confocal microscopic analysis of in vitro peritoneal macrophage phagocytosis of pHrodo E. coli (in red) after the addition (+ BMMC supernatants) or not (– BMMC supernatants) of cell-free supernatants from BMMCs activated for 15 minutes with fixed. Voronov I, Santerre JP, Hinek A et al () Macrophage phagocytosis of polyethylene particulate in vitro.

J Biomed Mater Res 40–51 PubMed CrossRef Google Scholar Author: Kang Jung Kim. Macrophage biology. Macrophages can exhibit both protective and pathogenic phenotypes in response to environmental cues. Evaluating these distinct functional phenotypes is essential for understanding host defense, auto-immunity, antitumor immunity as well as anti-inflammatory function.

Controlled induction of phagocytosis in macrophages offers the ability to therapeutically regulate the immune system as well as improve delivery of chemicals or biologicals for immune processing.

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Maximizing particle uptake by macrophages through Fc receptor-mediated phagocytosis could lead to new delivery mechanisms in drug or vaccine development. Fc ligand density and particle size were. THE EFFECTS OF POLYETHYLENE PARTICLE PHAGOCYTOSIS ON THE VIABILITY OF MATURE HUMAN MACROPHAGES BY PETER ASPINALL, KYRA BURNETT, JOE ultra high molecular weight polyethylene (UHMWPE) and were free of endotoxin phagocytosis on cell viability in vitro.

Ultimately determined HDPE particles increased cell viability. Plasma Membrane Synthesis in the Macrophage following Phagocytosis of Polystyrene Latex Particles* Isolation of Macrophage Plasma Membrane-Membranes were isolated by the Tris method of Warren et al. (10) modified for use with macrophages.

Macrophages grown in vitro for 24 hours were placed at 4” for 60 min to round the cells, rinsed once. In keeping with this idea, recent in vitro studies have confirmed high level resistance by S. aureus to neutrophil and macrophage mediated killing. Professional phagocytes play a key role in host defence by recognizing, engulfing, and killing microorganisms; yet only a small group of pathogens can persist inside these cells, evading host defences.

In-vitro Phagocytosis Assay of Macrophages:The term phagocytosis itself describes its mean phage = engulfment; cytosis: cell process. In other words, phagocytosis is the cellular process of engulfing solid particles by the cell membrane to form an internal phagosome, or.

Joint replacement has proven to be an extremely successful and cost-effective means of relieving arthritic pain and improving quality of life for recipients.

Wear debris-induced osteolysis is, however, a major limitation and causes orthopaedic implant aseptic loosening, and various cell types including macrophages, monocytes, osteoblasts, and osteoclasts, are involved. During the last few Cited by: The Natural Resistance Associated Macrophage Protein (NRAMP) is found on the surface of lysosomes of macrophages and migrates to the phagosome compartment after phagocytosis.

NRAMP plays an important role in controlling infection by removing iron and other divalent cations from the phagosomal environment (86, 87). 2 Differentiation of Human Monocytes In Vitro: A Model of Macrophage Ontogeny R. Andreesen and M. Kreutz Introduction 9 Mechanisms Generating Macrophage Heterogeneity: Monocyte Subpopulations and/or Microenvironment 11 Cell Lines as Model for Monocyte Differentiation 12 Monocyte Differentiation Induced by Serum An alveolar macrophage (or dust cell) is a type of macrophage, a professional phagocyte, found in the pulmonary alveoli, near the pneumocytes, but separated from the wall.

Activity of the alveolar macrophage is relatively high, because they are located at one of the major boundaries between the body and the outside world. Phagocytosis of particulate antigens (bacteria) can activate 2. Cytokines produced by TH enhance activity 3.

Mediators of inflammatory response (histamine) 4. Bacterial cell wall components 5. γ-interferon produced by TH cells.

Exposure to diesel exhaust particles (DEP) was shown to increase the susceptibility of the lung to bacterial infection in rats.

In this study, the effects of DEP on alveolar macrophage (AM) phagocytic and bactericidal functions and cytokine secretion by AM and lymphocytes in response to Listeria monocytogenes infection were investigated in vitro and the roles of different DEP components in.

Immediately after ingestion, the amount of the plasma membrane has descreased as judged by a reduction in cell volume, spreading, pinocytosis, and phagocytosis. During the next 6 hours, the phagolysosomal 5'-nucleotidase activity decreases with a half-life of about 2 hours.

Phagocytosis. Our laboratory has developed a flow cytometric assay to quantify alveolar macrophage (MФ) phagocytosis of bacteria within a live animal. MФs collected by bronchoalveolar lavage from rats infected transtracheally with Syto 9-labeled bacteria are fluorescently labeled for identification and analyzed by flow cytometry to.

The purpose of this study was to evaluate the effects of surface properties of bone implants coated with hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) on platelets and macrophages upon implant installation and compare them to grit-blasted Ti and Thermanox used as a control.

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Surface properties were characterized using scanning electron microscopy, profilometry, crystallography Cited by: 7. Start studying Bio - Exam 2. Learn vocabulary, terms, and more with flashcards, games, and other study tools.

Search. Tonsils have blind-ended structures called _____ that trap bacteria and particulate matter. a)germinal centers b)tonsillar corpuscles c)tonsillar crypts phagocytosis d)B cells. In Vitro Phagocytosis: Principle, Materials and Procedure In Vitro Phagocytosis: Principle, Materials and Procedure: A drop of whole blood is mixed with a drop of a bacterial culture and incubated at room temperature to demonstrate engulfment of bacteria by leukocytes.

PROPERTIES OF TISSUE SPECIFIC MACROPHAGES BEFORE AND AFTER IN VITRO ACTIVATION @IVAN^EVI]-SIMONOVI] [email protected]*, INI]-KANADA ALEKSANDRA**, STOJANOVI] MARIJANA** and DIMITRIJEVI] LJILJANA** *Institute of Pathophysiology, School of Medicine, University of Kragujevac, Kragujevac, **Institute of Immunology and Virology, Torlak, Belgrade (Received Estimation of the uptake potential of rabbit alveolar macrophages is performed in-vitro using P labeled bacteria, with the lytic potential of the same cells prior to phagocytosis estimated from the activity of six lysosomal hydrolase enzymes and related to macrophages as a means for .False Question 14 / pts Textbook Question: B cells are the principal cells of humoral immunity, whereas T cells are the principal cells of cell-mediated immunity.

Answer 1: Correct! B cells Answer 2: Correct! T cells Question 15 / pts In the Lymph nodes, paracortex contains B cells and neutrophils Correct! Tcells and antigen presenting cells Erythrocytes and basophils.